IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/22

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(Ligation)
Current revision (07:36, 17 September 2012) (view source)
(Colicin Assay)
 
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==Colicin Assay==
==Colicin Assay==
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[[Image:SGassayWTWT220812.jpg|300px]]
[[Image:SGassayWTWT220812.jpg|300px]]
[[Image:SGassayWTC2220812.jpg|300px]]
[[Image:SGassayWTC2220812.jpg|300px]]

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Colicin Assay

Ligation

Reagent pSG.024A pSG.024B PSG.002B -
Vector pSG.002A 1 uL pSG.002B 1.5 uL pSG.002B 1 uL
Insert pSG.013 3 uL pSG.013 uL -
Buffer 10x 1 uL 1 uL 1 uL
T4 DNA Ligase 0.5 uL 0.5 uL 0.5 uL
H2O 4.5 uL 4 uL 4.5 uL
Total 10 uL 10 uL 10 uL
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