IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/29

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Current revision (07:35, 17 September 2012) (view source)
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#Prepared competent NEB Turbo+pSG.008 (ColE2 Immune)
#Prepared competent NEB Turbo+pSG.008 (ColE2 Immune)
#Prepared Z1.08.23 cells +-IPTG and WT ColE2 for toxicity assay
#Prepared Z1.08.23 cells +-IPTG and WT ColE2 for toxicity assay

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  1. Prepared competent NEB Turbo+pSG.008 (ColE2 Immune)
  2. Prepared Z1.08.23 cells +-IPTG and WT ColE2 for toxicity assay
  3. Digested pSG.013-1, pSG.013-2, pSG.002, pSG.018 and gel purified
Reagent pSG.002 pSG.013-1 pSG.013-2 pSG.018
DNA 7 uL 20 uL 25 uL 6 uL
XbaI 2 uL 2 uL 2 uL -
SpeI - - - 2 uL
PstI 2 uL 2 uL 2 uL 2 uL
Buffer 10x 4 uL 4 uL 4 uL 4 uL
H2O 25 uL 12 uL 7 uL 26 uL
Total 40 uL 40 uL 40 uL 40 uL


Image:SGgel2908121.jpg

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