IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/09/18

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(Restriction Digest)
Current revision (17:32, 22 September 2012) (view source)
(Restriction Digest)
 
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==Restriction Digest==
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==Making Biobricks==
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===Restriction Digest===
[[Image: SGRD190912.jpg | 350 px]]
[[Image: SGRD190912.jpg | 350 px]]
[[Image: SGgel180912.jpg | 350 px]]
[[Image: SGgel180912.jpg | 350 px]]
[[Image: SGgel1809122.jpg | 360 px]]
[[Image: SGgel1809122.jpg | 360 px]]
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==PCR of pSG.013 and pSG.014==
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===Protocol===
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For each pSG.013 and pSG.014
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*H20 37uL
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*5x Phusion HF Buffer 10 uL
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*10 mM dNTPs mix 1 uL
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*primer mix (iSG.003+iSG.004 for pSG.013 and iSG.005+iSG.006 for pSG.014) 1 uL
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*template DNA (ColE2 plasmid) 0.5uL
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*Phusion DNA polymerase 0.5 uL
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Making Biobricks

Restriction Digest

PCR of pSG.013 and pSG.014

Protocol

For each pSG.013 and pSG.014

  • H20 37uL
  • 5x Phusion HF Buffer 10 uL
  • 10 mM dNTPs mix 1 uL
  • primer mix (iSG.003+iSG.004 for pSG.013 and iSG.005+iSG.006 for pSG.014) 1 uL
  • template DNA (ColE2 plasmid) 0.5uL
  • Phusion DNA polymerase 0.5 uL
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