IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/09/19: Difference between revisions
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#Incubate for 30 minutes. | #Incubate for 30 minutes. | ||
#Dilute 1e5x (1000x then 100x) in MgSO4 e-2M. | #Dilute 1e5x (1000x then 100x) in MgSO4 e-2M. | ||
#Plate | #Plate 40uL in Cm to kill the Colicin cells and leave the immune cells. | ||
Note: 1ml of cells of OD 1.0 has 1e9 cells, so we expect if all cells survive we will get 200 colonies at the end. | Note: 1ml of cells of OD 1.0 has 1e9 cells, so we expect if all cells survive we will get 200 colonies at the end. | ||
Revision as of 15:52, 23 September 2012
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LigationpLac + Colicin E2 Immunity CharacterizationWe want to characterize the immunity by varying the concentration of IPTG and the ColicinE2 cells. Protocol
Note: 1ml of cells of OD 1.0 has 1e9 cells, so we expect if all cells survive we will get 200 colonies at the end. ResultsA bit weird and random.. INSERT TABLE HERE
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