IGEM:Paris Bettencourt 2012/Previous Biosafety iGEM Projects: Difference between revisions
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| <center> [http://2011.igem.org/Team:Caltech Bioremediation of Endocrine Disruptors Using Genetically Modified Escherichia Coli] </center> | | <center> [http://2011.igem.org/Team:Caltech Bioremediation of Endocrine Disruptors Using Genetically Modified Escherichia Coli] </center> | ||
| Endocrine-disrupting chemicals (EDCs) are chemicals that interact with the endocrine system by binding to hormone receptors, causing problems in sexual development and reproduction of organisms | | Endocrine-disrupting chemicals (EDCs) are chemicals that interact with the endocrine system by binding to hormone receptors, causing problems in sexual development and reproduction of organisms | ||
| *Water filtration system for containment of microbes (filters all microbes, does not retain free DNA). | | Biosafety ideas: | ||
*Water filtration system for containment of microbes (filters all microbes, does not retain free DNA). | |||
*Use the ccdB gene (BBa_P1016 and BBa_P1010)on a plasmid in conjunction with an E. coli strain such as DB3.1 (BBa_V1005). The plasmid will code for the “death gene” which will kill any cell that does not code for immunity in its genome. If a native microorganism would uptake this man-made plasmid, it would die, preventing the propagation of the recombinant DNA in the environment (Featured Parts: Cell Death). | *Use the ccdB gene (BBa_P1016 and BBa_P1010)on a plasmid in conjunction with an E. coli strain such as DB3.1 (BBa_V1005). The plasmid will code for the “death gene” which will kill any cell that does not code for immunity in its genome. If a native microorganism would uptake this man-made plasmid, it would die, preventing the propagation of the recombinant DNA in the environment (Featured Parts: Cell Death). | ||
*Another similar “suicide” containment system uses streptavidin (BBa_J36841) (Kaplan, Mello et al. 1999; Urgun-Demirtas, Stark et al. 2006). This protein binds very tightly to biotin, a required co-enzyme for many metabolic pathways. This makes biotin unavailable and causes cell death. Kaplan et al. reported cell counts were reduced 99.9% in eight hours after their system was activated by absence of pollutant to degrade. | *Another similar “suicide” containment system uses streptavidin (BBa_J36841) (Kaplan, Mello et al. 1999; Urgun-Demirtas, Stark et al. 2006). This protein binds very tightly to biotin, a required co-enzyme for many metabolic pathways. This makes biotin unavailable and causes cell death. Kaplan et al. reported cell counts were reduced 99.9% in eight hours after their system was activated by absence of pollutant to degrade. |
Revision as of 02:34, 8 June 2012
Team | Year | Project Name | Project Summary | Biosafety Idea |
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Kill switch | Kill switch. Our kill switch is designed by inserting an antimicrobial peptide (AMP) gene into E.Coli | |||
Engineer bacteria to accelerate plant root development | Toxin/antitoxin. Consits of the insertion of the Holin + Endolysine and Anti Holin genes. | |||
Bacteria that detects and signals the presence of nitrates | Encapsulation in a gel. We encapsulated our bacteria in beeds made out of a non toxic gel. | |||
Detect chitin,and alert the plant by stimulating an early hypersensitive response against infection. | A few ideas but no design:
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Optimize production of terpenes in Saccharomyces cerevisiae yeast (to help trees fight invading beetles and fungi) | A few ideas (no design):
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Heavy metal bioreporter and bioabsorbent engineering | A few superficial ideas:
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Projects which could be useful for a space travelling:
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Not about biosafety, but:
They do not believe that their BioBrick parts will have any negative effect on the environment. But they could be interested by our project! | |||
CONTROLLING ICE FORMATION.
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Suicide mechanism: DNA nuclease.
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Biosensor to detect metals in waste water | One idea (no design):
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Decontamination of radioactive cobalt in water by a biofilm | Biofilm & Adherence =
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Sensing methane gas and converting it into methanol | Kill switch = Toxin temperature induced
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Engineer bacteria to allow them to transport packets of chemicals, and then form a network. | Promoter responsive to synthetic chemicals not present in nature. | |||
An easy way to allowing the insertion and/or deletion of genes in the E. coli chromosome in a minimal number of steps | FLP recombination to remove resistance gene, eg. FRT - Cm - FRT will remove the Cm resistance gene | |||
Endocrine-disrupting chemicals (EDCs) are chemicals that interact with the endocrine system by binding to hormone receptors, causing problems in sexual development and reproduction of organisms | Biosafety ideas:
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