IGEM:Paris Bettencourt 2012/Protocols/Biobrick Retrieval: Difference between revisions

Revision as of 05:39, 5 July 2012

This protocol can be used to retrieve biobrick DNA from kit plates. The resulting DNA solution can be used directly for transformation.

Find the correct Plate and well by locating the kit edition (year), plate number, and well coordinate (i.e. 2012 Kit Plate 4 Well 1A)
Look at your well from below to find the where the DNA is deposited. It is visible as a deposit of red solid at one of the corners (see image).
Punch a hole in the foil of your well using a 200 uL pipette tip. Push the foil to the sides.
Using another 200 uL pipette tip, carefully add 10 uL of distilled water to the well, on the DNA.
Gently scrape and mix the DNA. Pipette up and down.
Let the solution incubate in the well at least 5 minutes.
Transfer the 10 uL of DNA solution to a 1.5 mL centrifuge tube.
Store DNA in -20C.

@@ Line 1: / Line 1: @@
-[[File:Biobrick_deposit.png|thumb|left|alt=Red DNA stripe|Dry DNA and red dye is deposited along one corner of the well. Find the DNA's location before you add water.]]
+==Biobrick Retrieval==
+This protocol can be used to retrieve biobrick DNA from kit plates. The resulting DNA solution can be used directly for transformation.
+* Find the correct Plate and well by locating the kit edition (year), plate number, and well coordinate (i.e. 2012 Kit Plate 4 Well 1A)
+* Look at your well from below to find the where the DNA is deposited. It is visible as a deposit of red solid at one of the corners (see image).
+* Punch a hole in the foil of your well using a 200 uL pipette tip. Push the foil to the sides.
+* Using another 200 uL pipette tip, carefully add 10 uL of distilled water to the well, on the DNA.
+* Gently scrape and mix the DNA. Pipette up and down.
+* Let the solution incubate in the well at least 5 minutes.
+* Transfer the 10 uL of DNA solution to a 1.5 mL centrifuge tube.
+* Store DNA in -20C.
+[[Image:Biobrick_deposit.png|thumb|left|alt=Red DNA stripe|Dry DNA and red dye is deposited along one corner of the well. Find the DNA's location before you add water.]]