IGEM:Paris Bettencourt 2012/Protocols/Biobrick Retrieval

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Current revision (08:40, 5 July 2012) (view source)
 
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This protocol can be used to retrieve biobrick DNA from kit plates. The resulting DNA solution can be used directly for transformation.
This protocol can be used to retrieve biobrick DNA from kit plates. The resulting DNA solution can be used directly for transformation.
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[[Image:Biobrick_deposit.png|thumb|left|alt=Red DNA stripe|Dry DNA and red dye is deposited along one corner of the well. Find the DNA's location before you add water.]]
* Find the correct Plate and well by locating the kit edition (year), plate number, and well coordinate (i.e. 2012 Kit Plate 4 Well 1A)
* Find the correct Plate and well by locating the kit edition (year), plate number, and well coordinate (i.e. 2012 Kit Plate 4 Well 1A)
* Look at your well from below to find the where the DNA is deposited. It is visible as a deposit of red solid at one of the corners (see image).
* Look at your well from below to find the where the DNA is deposited. It is visible as a deposit of red solid at one of the corners (see image).
* Punch a hole in the foil of your well using a 200 uL pipette tip. Push the foil to the sides.
* Punch a hole in the foil of your well using a 200 uL pipette tip. Push the foil to the sides.
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* Using another 200 uL pipette tip, carefully add 10 uL of distilled water to the well, on the DNA.
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* Using another 200 uL pipette tip, carefully add 10 uL of distilled water to the well.
* Gently scrape and mix the DNA. Pipette up and down.
* Gently scrape and mix the DNA. Pipette up and down.
* Let the solution incubate in the well at least 5 minutes.
* Let the solution incubate in the well at least 5 minutes.
* Transfer the 10 uL of DNA solution to a 1.5 mL centrifuge tube.
* Transfer the 10 uL of DNA solution to a 1.5 mL centrifuge tube.
* Store DNA in -20C.
* Store DNA in -20C.
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[[Image:Biobrick_deposit.png|thumb|left|alt=Red DNA stripe|Dry DNA and red dye is deposited along one corner of the well. Find the DNA's location before you add water.]]
 

Current revision

Biobrick Retrieval

This protocol can be used to retrieve biobrick DNA from kit plates. The resulting DNA solution can be used directly for transformation.


Dry DNA and red dye is deposited along one corner of the well. Find the DNA's location before you add water.
Dry DNA and red dye is deposited along one corner of the well. Find the DNA's location before you add water.
  • Find the correct Plate and well by locating the kit edition (year), plate number, and well coordinate (i.e. 2012 Kit Plate 4 Well 1A)
  • Look at your well from below to find the where the DNA is deposited. It is visible as a deposit of red solid at one of the corners (see image).
  • Punch a hole in the foil of your well using a 200 uL pipette tip. Push the foil to the sides.
  • Using another 200 uL pipette tip, carefully add 10 uL of distilled water to the well.
  • Gently scrape and mix the DNA. Pipette up and down.
  • Let the solution incubate in the well at least 5 minutes.
  • Transfer the 10 uL of DNA solution to a 1.5 mL centrifuge tube.
  • Store DNA in -20C.
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