IGEM:Paris Bettencourt 2012/Protocols/Glycerol stock: Difference between revisions
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(New page: = Glycerol stocks= At every new bacteria you do 2 Glycerol Stocks of it. 750μL of the cell culture. 250 μL of 60% sterile glycerol. then: Freezer (-80°C and -20°C)) |
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At every new bacteria you do 2 Glycerol Stocks of it. | At every new bacteria you do 2 Glycerol Stocks of it. | ||
750μL of the cell culture. | *750μL of the cell culture. | ||
250 μL of 60% sterile glycerol. | *250 μL of 60% sterile glycerol. | ||
then: Freezer (-80°C and -20°C) | then: Freezer (-80°C and -20°C) | ||
Latest revision as of 01:29, 6 July 2012
Glycerol stocks
At every new bacteria you do 2 Glycerol Stocks of it.
- 750μL of the cell culture.
- 250 μL of 60% sterile glycerol.
then: Freezer (-80°C and -20°C)
