IGEM:Paris Bettencourt 2012/Protocols/Plates: Difference between revisions
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= Protocol = | = LB Agar (LBA) Protocol (500ml) = | ||
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* Melt LBA in microwave (~8 min). | |||
* Cool LBA before you can touch it without burn your skin (~4 min in water bath). | |||
* Add required chemicals (antibiotic, etc). | |||
* Add 24 ml of LBA into each plate. | |||
* Wait wile plates will be thicken. |
Revision as of 08:16, 30 June 2012
Plate color codes
LB Agar (LBA) Protocol (500ml)
- Melt LBA in microwave (~8 min).
- Cool LBA before you can touch it without burn your skin (~4 min in water bath).
- Add required chemicals (antibiotic, etc).
- Add 24 ml of LBA into each plate.
- Wait wile plates will be thicken.