IGEM:Paris Bettencourt 2012/Protocols/Plates: Difference between revisions

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= Protocol =
= Plate color codes =


= Antibiotics =
{| class="wikitable" width="100%" |
 
! width="20%" | <center>Antibiotic type</center>
{| class="wikitable"
! width="20%" | <center>Color code</center>
|-
! width="20%" | <center>Dilutant</center>
! <center>Antibiotic type</center>
! width="20%" | <center>Stock concentration</center>
! <center>Color code</center>
! width="20%" | <center>Dilution</center>
! <center>Dilutant</center>
! <center>Stock concentration</center>
! <center>Dilution</center>
|-
|-
| <center>Plain</center>
| <center>Plain</center>
| style="background:black; color:white" |  
| style="background:black; color:white" | <center>black</center>
| <center><nowiki>-</nowiki></center>
| <center><nowiki>-</nowiki></center>
| <center><nowiki>-</nowiki></center>
| <center><nowiki>-</nowiki></center>
Line 18: Line 15:
|-
|-
| <center>Ampicilline</center>
| <center>Ampicilline</center>
| style="background:red; color:white" |  
| style="background:red; color:white" | <center>red</center>
| <center>H2O + filtration</center>
| <center><math>H_{2}O</math> + filtration</center>
| <center>100 mg/ml</center>
| <center> 100 mg/ml </center>
| <center>x1000</center>
| <center>x1000</center>
|-
|-
| <center>Chloramphenicol</center>
| <center>Chloramphenicol</center>
| style="background:blue; color:white" |  
| style="background:blue; color:white" | <center>blue</center>
| <center>ethanol 100%</center>
| <center>ethanol 100%</center>
| <center>30 mg/ml</center>
| <center> 30 mg/ml </center>
| <center>x1000</center>
| <center>x1000</center>
|-
|-
| <center>Kanamycine</center>
| <center>Kanamycine</center>
| style="background:green; color:white" |  
| style="background:green; color:white" | <center>green</center>
| <center>H2O + filtration</center>
| <center><math>H_{2}O</math> + filtration</center>
| <center>100 mg/ml</center>
| <center> 100 mg/ml </center>
| <center>x1000</center>
| <center>x1000</center>
|-
|-
| <center>Spectinomycine</center>
| <center>Spectinomycine</center>
| style="background:orange; color:white" |  
| style="background:orange; color:white" | <center>orange</center>
| <center>H2O + filtration</center>
| <center><math>H_{2}O</math> + filtration</center>
| <center>25 mg/ml</center>
| <center> 25 mg/ml </center>
| <center>x500/333</center>
| <center>x500/333</center>
|-
|-
|}
|}
= LB Agar (LBA) Protocol (500ml) =
* Melt LBA in microwave (~8 min).
* Cool LBA before you can touch it without burn your skin (~4 min in water bath).
* Add required chemicals (antibiotic, etc).
* Add 24 ml of LBA into each plate.
* Wait while plates will be thicken.
* Dry them 45' '''under the flow hood''', with the lid open.

Latest revision as of 02:51, 4 July 2012

Plate color codes

Antibiotic type
Color code
Dilutant
Stock concentration
Dilution
Plain
black
-
-
-
Ampicilline
red
[math]\displaystyle{ H_{2}O }[/math] + filtration
100 mg/ml
x1000
Chloramphenicol
blue
ethanol 100%
30 mg/ml
x1000
Kanamycine
green
[math]\displaystyle{ H_{2}O }[/math] + filtration
100 mg/ml
x1000
Spectinomycine
orange
[math]\displaystyle{ H_{2}O }[/math] + filtration
25 mg/ml
x500/333

LB Agar (LBA) Protocol (500ml)

  • Melt LBA in microwave (~8 min).
  • Cool LBA before you can touch it without burn your skin (~4 min in water bath).
  • Add required chemicals (antibiotic, etc).
  • Add 24 ml of LBA into each plate.
  • Wait while plates will be thicken.
  • Dry them 45' under the flow hood, with the lid open.
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