Latest revision as of 02:51, 4 July 2012

Plate color codes

Antibiotic type	Color code	Dilutant	Stock concentration	Dilution
Plain	black	-	-	-
Ampicilline	red	[math]\displaystyle{ H_{2}O }[/math] + filtration	100 mg/ml	x1000
Chloramphenicol	blue	ethanol 100%	30 mg/ml	x1000
Kanamycine	green	[math]\displaystyle{ H_{2}O }[/math] + filtration	100 mg/ml	x1000
Spectinomycine	orange	[math]\displaystyle{ H_{2}O }[/math] + filtration	25 mg/ml	x500/333

@@ Line 2: / Line 2: @@
 {| class="wikitable" width="100%" |
-! width="20%" |<center>Antibiotic type</center>
+! width="20%" | <center>Antibiotic type</center>
 ! width="20%" | <center>Color code</center>
 ! width="20%" | <center>Dilutant</center>
@@ Line 40: / Line 40: @@
 |}
-= Protocol =
+= LB Agar (LBA) Protocol (500ml) =
-* Melt LBA in microwave (8min)
+* Melt LBA in microwave (~8 min).
-*
+* Cool LBA before you can touch it without burn your skin (~4 min in water bath).
-*
+* Add required chemicals (antibiotic, etc).
-*
+* Add 24 ml of LBA into each plate.
+* Wait while plates will be thicken.
+* Dry them 45' '''under the flow hood''', with the lid open.