IGEM:Paris Bettencourt 2012/Protocols/Plates: Difference between revisions
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= LB Agar (LBA) Protocol (500ml) = | = LB Agar (LBA) Protocol (500ml) = | ||
* Melt LBA in microwave (~8 min). | * Melt LBA in microwave (~8 min). | ||
Line 50: | Line 46: | ||
* Add required chemicals (antibiotic, etc). | * Add required chemicals (antibiotic, etc). | ||
* Add 24 ml of LBA into each plate. | * Add 24 ml of LBA into each plate. | ||
* Wait | * Wait while plates will be thicken. | ||
* Dry them 45' '''under the flow hood''', with the lid open. |
Latest revision as of 02:51, 4 July 2012
Plate color codes
LB Agar (LBA) Protocol (500ml)
- Melt LBA in microwave (~8 min).
- Cool LBA before you can touch it without burn your skin (~4 min in water bath).
- Add required chemicals (antibiotic, etc).
- Add 24 ml of LBA into each plate.
- Wait while plates will be thicken.
- Dry them 45' under the flow hood, with the lid open.