IGEM:Paris Bettencourt 2012/Protocols/Plates: Difference between revisions

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Revision as of 07:40, 29 June 2012

Plate color codes

Antibiotic type
Color code
Dilutant
Stock concentration
Dilution
Plain
black
-
-
-
Ampicilline
red
[math]\displaystyle{ H_{2}O }[/math] + filtration
100 mg/ml
x1000
Chloramphenicol
blue
ethanol 100%
30 mg/ml
x1000
Kanamycine
green
[math]\displaystyle{ H_{2}O }[/math] + filtration
100 mg/ml
x1000
Spectinomycine
orange
[math]\displaystyle{ H_{2}O }[/math] + filtration
25 mg/ml
x500/333

Protocol

  • Melt LBA in microwave (8min)