IGEM:Paris Bettencourt 2012/Protocols/Plates

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< IGEM:Paris Bettencourt 2012 | Protocols
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Plate color codes

Antibiotic type
Color code
Dilutant
Stock concentration
Dilution
Plain
black
-
-
-
Ampicilline
red
H2O + filtration
100 mg/ml
x1000
Chloramphenicol
blue
ethanol 100%
30 mg/ml
x1000
Kanamycine
green
H2O + filtration
100 mg/ml
x1000
Spectinomycine
orange
H2O + filtration
25 mg/ml
x500/333

LB Agar (LBA) Protocol (500ml)

  • Melt LBA in microwave (~8 min).
  • Cool LBA before you can touch it without burn your skin (~4 min in water bath).
  • Add required chemicals (antibiotic, etc).
  • Add 24 ml of LBA into each plate.
  • Wait while plates will be thicken.
  • Dry them 45' under the flow hood, with the lid open.
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