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Plate color codes
| Antibiotic type
|
Color code
|
Dilutant
|
Stock concentration
|
Dilution
|
| Plain
|
black
|
-
|
-
|
-
|
| Ampicilline
|
red
|
[math]\displaystyle{ H_{2}O }[/math] + filtration
|
100 mg/ml
|
x1000
|
| Chloramphenicol
|
blue
|
ethanol 100%
|
30 mg/ml
|
x1000
|
| Kanamycine
|
green
|
[math]\displaystyle{ H_{2}O }[/math] + filtration
|
100 mg/ml
|
x1000
|
| Spectinomycine
|
orange
|
[math]\displaystyle{ H_{2}O }[/math] + filtration
|
25 mg/ml
|
x500/333
|
LB Agar (LBA) Protocol (500ml)
- Melt LBA in microwave (~8 min).
- Cool LBA before you can touch it without burn your skin (~4 min in water bath).
- Add required chemicals (antibiotic, etc).
- Add 24 ml of LBA into each plate.
- Wait while plates will be thicken.
- Dry them 45' under the flow hood, with the lid open.
