Stéphane
Gel electrophoresis (agarose 1,0% w/v, EtB) Verification of the DNA quantity at each step from the last batch (minipreps, restriction and ligation of 07/15).
- 6 µL DNA ladder (C#83 µg/mL)
- 5 µL pLux (R0062) digest with SpeI and PstI + 1 µL loading buffer
- nothing
- 5 µL pLux (R0062) after gel extraction + 1 µL loading buffer
- 5 µL RBS/GFP/term (E0240) digest with XbaI and PstI + 1 µL loading buffer
- nothing
- 5 µL RBS/GFP/term (E0240) after gel extraction + 1 µL loading buffer
- 5 µL pLux - RBS/GFP/term ligation + 1 µL loading buffer
Overnight culture
In 10ml LB and 10 µL corresponding antibiotic
- attC - Kan
- term-attC-mRFP - Kan
- term (B0015) - Amp
- RBS/GFP/term (E0240) - Amp
- RBS/LuxR (J37033) - Amp
- pLux (R0062) - Amp
- RBS/LuxI (K081008) - Amp
- Pstrong (J23119) - Amp
- Pmedium (J23110) - Amp
- kanR (P1003) - Amp
|