IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/22: Difference between revisions
No edit summary |
No edit summary |
||
(5 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Memo cell</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
|- | |- | ||
Line 28: | Line 28: | ||
*P1003 LB+Amp+Kan | *P1003 LB+Amp+Kan | ||
*YejF KO (from KEIO collection , KanR disrupt YejF gene) (LB+ Kan)*2 (one for glycerol) | *YejF KO (from KEIO collection , KanR disrupt YejF gene) (LB+ Kan)*2 (one for glycerol) | ||
|- | |||
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Population counter</span> | |||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |||
|- | |||
| colspan="2"| | |||
<br><font color=red>Aleksandra, Stéphane, Raphaël</font> | <br><font color=red>Aleksandra, Stéphane, Raphaël</font> | ||
<br><u>Minipreps</u> : 60 minipreps in total, 4 minipreps of a 5ml culture per biobrick, eluted with | <br><u>Minipreps</u> : 60 minipreps in total, 4 minipreps of a 5ml culture per biobrick, eluted with 30μL buffer and collected into 1 tube for a total volume of around 120μL: | ||
* I732006 (LacZ) | * I732006 (LacZ) | ||
* B0031 (weak RBS) | * B0031 (weak RBS) | ||
Line 100: | Line 106: | ||
<br><u>Gel electrophoresis (agarose 0.8% w/v), 100V for 30 mins</u> | <br><u>Gel electrophoresis (agarose 0.8% w/v), 100V for 30 mins</u> | ||
<br>This time, gel electrophoresis is used only for the biobricks | <br>This time, gel electrophoresis is used only for the biobricks supposed to be inserts in our constructions, as the cut and the uncut vector biobricks are inseparable on the gel (only several bp are cut out). | ||
We load all the restriction reaction's product into the wells (+6x loading buffer): | We load all the restriction reaction's product into the wells (+6x loading buffer): | ||
*TetA(C), 30μL + 5μL lb | *TetA(C), 30μL + 5μL lb | ||
Line 115: | Line 121: | ||
*LacZalpha, 30μL + 6μL lb | *LacZalpha, 30μL + 6μL lb | ||
[[Image:restr2207.jpg]] | [[Image:restr2207.jpg]] | ||
[[Image:restr22207.jpg]] | |||
<br> The LacZ alpha is too small and already migrated off the gel, so we couldn't obtain it. The RBS/LuxR biobrick (J370033) still seems to be not working, even though this sample was taken from the 2007 distribution. | <br> The LacZ alpha is too small and already migrated off the gel, so we couldn't obtain it. The RBS/LuxR biobrick (J370033) still seems to be not working, even though this sample was taken from the 2007 distribution. | ||
Revision as of 06:31, 29 July 2010
Memo cell | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antoine
3μL of 1kb Ladder picture of the gel
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Population counter | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|