IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/22

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(Autocreate 2010/07/22 Entry for IGEM:Paris_Liliane_Bettencourt/Notebook/Paris_2010)
(Entry title)
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<font color=red>Antoine</font>
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<br><u>Résults transformation biobrick R0040/C0040/R0011/C0012</u>
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Positif for all
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<br><u>Minipreps</u>
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pBM43
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From 10 ml of overnight culture to 60μL Vf.
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<br><u>electrophoresis Minipreps</u>
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5μL of each in wells.
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3μL of 1kb Ladder
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picture of the gel
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<br><u>Overnight culture </u>
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(biobrick R0040/C0040/R0011/C0012 in LB + Amp)*2 (one for glycerol)
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*P1003 LB+Amp+Kan
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*YejF KO (from KEIO collection , KanR disrupt YejF gene) (LB+ Kan)*2 (one for glycerol)
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Revision as of 14:21, 22 July 2010

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Antoine


Résults transformation biobrick R0040/C0040/R0011/C0012 Positif for all


Minipreps pBM43 From 10 ml of overnight culture to 60μL Vf.


electrophoresis Minipreps 5μL of each in wells.

3μL of 1kb Ladder

picture of the gel



Overnight culture (biobrick R0040/C0040/R0011/C0012 in LB + Amp)*2 (one for glycerol)

  • P1003 LB+Amp+Kan
  • YejF KO (from KEIO collection , KanR disrupt YejF gene) (LB+ Kan)*2 (one for glycerol)


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