|
Antoine
Résults transformation biobrick R0040/C0040/R0011/C0012
Positif for all
Minipreps
pBM43
From 10 ml of overnight culture to 60μL Vf.
electrophoresis Minipreps
5μL of each in wells.
3μL of 1kb Ladder
picture of the gel
Overnight culture
(biobrick R0040/C0040/R0011/C0012 in LB + Amp)*2 (one for glycerol)
- P1003 LB+Amp+Kan
- YejF KO (from KEIO collection , KanR disrupt YejF gene) (LB+ Kan)*2 (one for glycerol)
Aleksandra, Stéphane, Raphaël
Minipreps : 60 minipreps in total, 4 minipreps of a 5ml culture per biobrick, eluted with 50μL buffer and collected into 1 tube for a total volume of around 120μL:
- J732006 (LacZ)
- B0031 (weak RBS)
- B0032 (medium RBS)
- B0034 (standard RBS)
- J31007 (TetA C)
- C0062 (LuxR w/o RBS)
- J37033 (RBS/LuxR) from the 2007 distribution plate
- 23119 (Ps)
- J23110 (Pm)
- attC
- term/attC/mRFP
- B0015 (term)
- E0240 (RBS/GFP/term)
- K081008 (LuxI)
- R0062 (pLux)
Nanodrop to determine the amount of DNA in the miniprep samples:
| Sample name |
260/280 |
260/230 |
ng/μL
|
| term (B0015) |
1.82 |
2.34 |
119.9
|
| attC |
1.72 |
2.21 |
26.8
|
| RBS/LuxI (K081008) |
1.85 |
2.35 |
102.3
|
| Pmed (J23110) |
1.87 |
2.34 |
194.2
|
| Pstr (J23119) |
1.86 |
2.31 |
169.5
|
| LuxR (C0062) |
1.86 |
2.41 |
188.0
|
| TetA(C) (J31007) |
1.86 |
2.35 |
149.1
|
| RBS/LuxR (J37033) |
1.81 |
2.28 |
114.4
|
| stand.RBS (B0034) |
1.86 |
2.30 |
145.2
|
| med.RBS (B0032) |
1.90 |
2.34 |
120.1
|
| weakRBS (B0031) |
1.86 |
2.32 |
163.2
|
| LacZalpha (I732006) |
1.86 |
2.42 |
221.3
|
| pLux (R0062) |
1.93 |
2.27 |
66.6
|
| t/attC/mRFP |
1.97 |
2.13 |
33.1
|
| RBS/GFP/term (E0240) |
1.89 |
2.39 |
120.6
|
|
Population counter
