IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/23: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Memo cell</span> | |||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |||
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| colspan="2"| | |||
<font color=red>Antoine</font> | |||
<br><u>Minipreps</u> | |||
<br><u>Nanodrop</u> to determine the amount of DNA in the miniprep samples: | |||
{| | |||
|Sample name || 260/280 ||260/230 ||ng/μL | |||
|- | |||
| R0011 ||1.18 ||2.22||63 | |||
|- | |||
| C0012 ||1.89||2.39 ||154.5 | |||
|- | |||
| C0040 ||1.86 ||2.40||320.4 | |||
|- | |||
| R0040 ||1.86||2.31||106.5 | |||
|- | |||
| P1003 ||1.89 ||2.38||499.7 | |||
|} | |||
<br><u>Glycerols 20% -20/-80 °C</u> | |||
R0011 | |||
C0012 | |||
R0040 | |||
C0040 | |||
<br><u>Phage production</u> | |||
Infection with the phage P1 of a mutant for YejF gene from the Keio collection. | |||
|- | |- | ||
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Population counter</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Population counter</span> | ||
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*ladder | *ladder | ||
[[Image:gel2307_1.jpg]] | [[Image:gel2307_1.jpg]][[Image:gel2307_2.jpg]] | ||
<br> Agarose gel 3%, 50V to separate the small inserts | <br> Agarose gel 3%, 50V to separate the small inserts | ||
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*RBS/LuxI (K081008) : 40µl + 8µl lb | *RBS/LuxI (K081008) : 40µl + 8µl lb | ||
*RBS/LuxI (K081008) : 40µl + 8µl lb | *RBS/LuxI (K081008) : 40µl + 8µl lb | ||
[[Image: | [[Image:gel23072_1.jpg]] [[Image:gel23072_2.jpg]] | ||
<br><font color=red>Raphaël</font> | <br><font color=red>Raphaël</font> |
Revision as of 03:24, 2 August 2010
Memo cell | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Antoine
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Population counter | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Théotime
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