IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/24: Difference between revisions
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*TetA(C) (J31007) | *TetA(C) (J31007) | ||
br><u>Restriction digest</u> of the same minipreps as yestarday (concentration measured by nanodrop 07/23), around 3µg of DNA per reaction. | <br><u>Restriction digest</u> of the same minipreps as yestarday (concentration measured by nanodrop 07/23), around 3µg of DNA per reaction. | ||
*term/attC/mRFP : 42µl DNA + 5µl Buffer 3 10x + 0.5µl BSA 100x + 2µl XbaI + 2µl PstI | *term/attC/mRFP : 42µl DNA + 5µl Buffer 3 10x + 0.5µl BSA 100x + 2µl XbaI + 2µl PstI | ||
*attC/term/attC/mRFP : 42µl DNA + 5µl Buffer 3 10x + 0.5µl BSA 100x + 2µl XbaI + 2µl PstI | *attC/term/attC/mRFP : 42µl DNA + 5µl Buffer 3 10x + 0.5µl BSA 100x + 2µl XbaI + 2µl PstI |
Revision as of 15:33, 25 July 2010
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The absence of the band corresponding to the insert for attC/term/attC/mRFP raises some questions about the real nature of this plasmid...
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