IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/25: Difference between revisions

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{|{{table}} width="800"
{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Population counter</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
<br><font color=red>Raphaël</font>
* Insert your content here.
<br><u>Glycerol 15%</u>
2 glycerols per culture, one stored at -20°C and the other at -80°C
*TOP10 pSB1A2 weak RBS -TetA(C) - Amp
*TOP10 pSB1A2 med.RBS -TetA(C) - Amp
*TOP10 pSB1A2 weak RBS -LuxR - Amp
*TOP10 pSB1A2 med.RBS -LuxR - Amp
*TOP10 pSB1A2 stand.RBS -LuxR - Amp


<br><u>Minipreps</u> : 4 minipreps of a 10ml culture per biobrick, eluted with 30μL buffer and collected into 1 tube for a total volume of around 120μL:
*weak RBS -TetA(C) - Amp
*med.RBS -TetA(C) - Amp
*weak RBS -LuxR - Amp
*med.RBS -LuxR - Amp
*stand.RBS -LuxR - Amp
<br><u>Nanodrop</u> to determine the amount of DNA in the miniprep samples:
{|
|Sample name || 260/280 ||260/230 ||ng/μL
|-
|  weak RBS -TetA(C) ||1.87||2.31||186.5
|-
| med.RBS -TetA(C) ||1.86 ||2.31||185.1
|-
| weak RBS -LuxR ||1.84||2.38||159.8
|-
|  med.RBS -LuxR ||1.84 ||2.36 ||175.6
|-
|  stand.RBS -LuxR ||1.85 ||2.35 ||191.7
|}




Revision as of 15:48, 25 July 2010

Population counter <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Raphaël
Glycerol 15% 2 glycerols per culture, one stored at -20°C and the other at -80°C

  • TOP10 pSB1A2 weak RBS -TetA(C) - Amp
  • TOP10 pSB1A2 med.RBS -TetA(C) - Amp
  • TOP10 pSB1A2 weak RBS -LuxR - Amp
  • TOP10 pSB1A2 med.RBS -LuxR - Amp
  • TOP10 pSB1A2 stand.RBS -LuxR - Amp


Minipreps : 4 minipreps of a 10ml culture per biobrick, eluted with 30μL buffer and collected into 1 tube for a total volume of around 120μL:

  • weak RBS -TetA(C) - Amp
  • med.RBS -TetA(C) - Amp
  • weak RBS -LuxR - Amp
  • med.RBS -LuxR - Amp
  • stand.RBS -LuxR - Amp


Nanodrop to determine the amount of DNA in the miniprep samples:

Sample name 260/280 260/230 ng/μL
weak RBS -TetA(C) 1.87 2.31 186.5
med.RBS -TetA(C) 1.86 2.31 185.1
weak RBS -LuxR 1.84 2.38 159.8
med.RBS -LuxR 1.84 2.36 175.6
stand.RBS -LuxR 1.85 2.35 191.7



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