IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/25: Difference between revisions

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Revision as of 03:26, 2 August 2010

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Raphaël
Glycerol 15% 2 glycerols per culture, one stored at -20°C and the other at -80°C

  • TOP10 pSB1A2 weak RBS -TetA(C) - Amp
  • TOP10 pSB1A2 med.RBS -TetA(C) - Amp
  • TOP10 pSB1A2 weak RBS -LuxR - Amp
  • TOP10 pSB1A2 med.RBS -LuxR - Amp
  • TOP10 pSB1A2 stand.RBS -LuxR - Amp


Minipreps : 4 minipreps of a 10ml culture per biobrick, eluted with 30μL buffer and collected into 1 tube for a total volume of around 120μL:

  • weak RBS -TetA(C) - Amp
  • med.RBS -TetA(C) - Amp
  • weak RBS -LuxR - Amp
  • med.RBS -LuxR - Amp
  • stand.RBS -LuxR - Amp


Nanodrop to determine the amount of DNA in the miniprep samples:

Sample name 260/280 260/230 ng/μL
weak RBS -TetA(C) 1.87 2.31 186.5
med.RBS -TetA(C) 1.86 2.31 185.1
weak RBS -LuxR 1.84 2.38 159.8
med.RBS -LuxR 1.84 2.36 175.6
stand.RBS -LuxR 1.85 2.35 191.7



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