IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/26: Difference between revisions
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*strong RBS-LuxR | *strong RBS-LuxR | ||
[[Image:gel2307.jpg]] | [[Image:gel2307.jpg]][[Image:gel23072.jpg]] | ||
<br>The transformations from 07/23 have worked, we have the inserts of the expected size : 1.2kb for RBS+TetA(C) and 0.8kb for RBS+LuxR. We can purify these inserts to use them in further ligations. | <br>The transformations from 07/23 have worked, we have the inserts of the expected size : 1.2kb for RBS+TetA(C) and 0.8kb for RBS+LuxR. We can purify these inserts to use them in further ligations. | ||
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*pLux-GFP (ligation product purified by Aleksandra and Stéphane) | *pLux-GFP (ligation product purified by Aleksandra and Stéphane) | ||
[[Image:gel2607_2.jpg]] | [[Image:gel2607_2.jpg]][[Image:gel2607_22.jpg]] | ||
<br><u>Gel electrophoresis (3.0% agarose w/v, 50V)</u> | <br><u>Gel electrophoresis (3.0% agarose w/v, 50V)</u> | ||
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*LuxI | *LuxI | ||
[[Image:gel2607_3.jpg]] | [[Image:gel2607_3.jpg]] [[Image:gel2607_32.jpg]] | ||
<br>All of the restictions worked (except for the verification of the ligation pLux-GFP). | <br>All of the restictions worked (except for the verification of the ligation pLux-GFP). |
Revision as of 06:44, 29 July 2010
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Stéphane and Aleksandra
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