IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/26: Difference between revisions
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<font color=red>Antoine</font> | |||
Electrophoresis of digest products | |||
<br><u>Restriction digest</u> | |||
{| | |||
|Sample name||DNA (3ug)||enzyme||Buffer 2||H2O||BSA | |||
|- | |||
|C0040 || 9.4 ||1 X/P ||2.5||10.85||0.25 | |||
|- | |||
|C0012 || 19.4 ||1 X/P ||2.5||0.85||0.25 | |||
|- | |||
|B0015 || 20.25||1 X/P ||2.5||0||0.25 | |||
|- | |||
|J23110 || 20.25||1 S/P ||2.5||0||0.25 | |||
|- | |||
|P1003 || 6||1 S/P ||2.5||14.25||0.25 | |||
|} | |||
|- | |- | ||
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Population counter </span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Population counter </span> | ||
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*4µl strRBS/LuxR + 2.7µl Pstr + 1µl buffer + 0.5µl ligase + 1.8µl water | *4µl strRBS/LuxR + 2.7µl Pstr + 1µl buffer + 0.5µl ligase + 1.8µl water | ||
*13µl LacZ + 0.7µl weak RBS + 2µl buffer + 0.5µl ligase + 3.8µl water | *13µl LacZ + 0.7µl weak RBS + 2µl buffer + 0.5µl ligase + 3.8µl water | ||
*13µl LacZ + 0.9µl | *13µl LacZ + 0.9µl stand RBS + 2µl buffer + 0.5µl ligase + 3.6µl water | ||
*15µl LuxI + 2.9µl weak RBS + 2µl buffer + 0.5µl ligase | *15µl LuxI + 2.9µl weak RBS + 2µl buffer + 0.5µl ligase | ||
Revision as of 07:57, 2 August 2010
Memo-Cell | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antoine Electrophoresis of digest products
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Population counter | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Stéphane and Aleksandra
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