IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/07

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Théotime
Glycerol 15%, -20°C and -80°C stock per pop.

  • Pm/weakRBS/LuxR/attC/term/attC/RFP (1-2-3)
  • Ps/weakRBS/LuxR/attC/term/attC/RFP (1-2)
  • Ps/medRBS/LuxR/attC/term/attC/RFP (1-2) (error on gly.2 -> Media is clear, there's no bacteria, I didn't see)
  • Pm/stdRBS/LuxR/attC/term/attC/RFP (1-2)
  • Ps/RFP (1-2-3-4)


Xavier
MiniPrep in 40 µL water.

  • Pm/weakRBS/LuxR/attC/term/attC/RFP (1-2-3)
  • Ps/weakRBS/LuxR/attC/term/attC/RFP (1-2)
  • Ps/medRBS/LuxR/attC/term/attC/RFP (1)
  • Pm/stdRBS/LuxR/attC/term/attC/RFP (1-2)


Théotime
Miniprep in 40µL water.

  • Ps/RFP (1-2-3-4)


Nanodrop
Set values here.

Restriction Digest with Xba1 (1µL) and Pst1(1µL), in 2.5µL Buffer 3, 0.25µL BSA and 5µL water for 15µL miniprep.
(Note : Total=25µL, but I got ~30µL, I've made an error in my manip ...)

  • Pm/weakRBS/LuxR/attC/term/attC/RFP (1-2-3)
  • Ps/weakRBS/LuxR/attC/term/attC/RFP (1-2)
  • Ps/medRBS/LuxR/attC/term/attC/RFP (1)
  • Pm/stdRBS/LuxR/attC/term/attC/RFP (1-2)


Gel Electro with 50µL tBE and 0.75g Agarose (1.5%) and 1.2µL BET, in tBE 0.5X.

  • Pm/weakRBS/LuxR/attC/term/attC/RFP (1-2-3)
  • Ps/weakRBS/LuxR/attC/term/attC/RFP (1-2)
  • Ps/medRBS/LuxR/attC/term/attC/RFP (1)
  • Pm/stdRBS/LuxR/attC/term/attC/RFP (1-2)
  • empty
  • empty
  • 1kb ladder


The dye forms a wave... I've stop the gel when 1kb ladder touch the last line, but the "wave" don't.


Culture Overnight in 12mL AMP.

  • K081014
  • I13507
  • Ps/medRBS/attC/term/attC/RFP (3)
  • Pm/weakRBS/attC/term/attC/RFP (4)
  • Ps/stdRBS/attC/term/attC/RFP (2)


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