IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/09/05: Difference between revisions

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<br><u>Touchdown PCR</u> of the 13 minipreps from yesterday and also of 6 other constructs (elongation time 6 mins), then gel electrophoresis.  
<br><u>Touchdown PCR</u> of the 13 minipreps from yesterday and also of 6 other constructs (elongation time 6 mins), then gel electrophoresis.  


<br><u>Gel 1</u>
<br><u>Gel 1 (PCR)</u>
<br>Minipreps from 31/08
<br>Minipreps from 31/08
*1 Pms/wTet II
*1 Pms/wTet II
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[[Image:PCR0509.jpg]]
[[Image:PCR0509.jpg]]


<br><u>Gel 2</u>
<br><u>Gel 2 (PCR)</u>
<br>Minipreps from yesterday (04/05)
<br>Minipreps from yesterday (04/05)
*17, 18 Pss/sLac/LuxI III, 2 cultures
*17, 18 Pss/sLac/LuxI III, 2 cultures
Line 36: Line 36:


<br>→ Colonies 7, 9, 10, 17, 18, 19 seem to be of a right size
<br>→ Colonies 7, 9, 10, 17, 18, 19 seem to be of a right size
<br><u>Restriction digest and gel electrophoresis</u> of yesterday's minipreps with E+P
*7,8 Pms/mTet/LuxI, 2 cultures
*9,10 Pss/wTet/LuxI, 2 cultures
*11,12 Psm/sTet/LuxI, 2 cultures
*13,14 Psm/wTetIII/I13507/pLux/GFP, 2 cultures
*15,16 Psm/wTetII/I13507/pLux/GFP, 2 cultures
*17, 18 Pss/sLac/LuxI III, 2 cultures
*19 Pms/sTet/LuxI (on the PCR gel, see above)
[[Image:gel0509_2.jpg]]


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Revision as of 04:33, 14 September 2010

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Aleksandra
Touchdown PCR of the 13 minipreps from yesterday and also of 6 other constructs (elongation time 6 mins), then gel electrophoresis.


Gel 1 (PCR)
Minipreps from 31/08

  • 1 Pms/wTet II
  • 2 Pms/mTet III
  • 3 Psm/mTet III
  • 4 Psw/mTet III
  • 5 Pss/wTet II
  • 6 Psw/wTet II

Minipreps from yesterday (04/05)

  • 7,8 Pms/mTet/LuxI, 2 cultures
  • 9,10 Pss/wTet/LuxI, 2 cultures
  • 11,12 Psm/sTet/LuxI, 2 cultures
  • 13,14 Psm/wTetIII/I13507/pLux/GFP, 2 cultures
  • 15,16 Psm/wTetII/I13507/pLux/GFP, 2 cultures
  • Ladder 1 kb


Gel 2 (PCR)
Minipreps from yesterday (04/05)

  • 17, 18 Pss/sLac/LuxI III, 2 cultures
  • 19 Pms/sTet/LuxI

Digestion from tod ay(see further)

  • 19 Pms/sTet/LuxI


→ Colonies 7, 9, 10, 17, 18, 19 seem to be of a right size


Restriction digest and gel electrophoresis of yesterday's minipreps with E+P

  • 7,8 Pms/mTet/LuxI, 2 cultures
  • 9,10 Pss/wTet/LuxI, 2 cultures
  • 11,12 Psm/sTet/LuxI, 2 cultures
  • 13,14 Psm/wTetIII/I13507/pLux/GFP, 2 cultures
  • 15,16 Psm/wTetII/I13507/pLux/GFP, 2 cultures
  • 17, 18 Pss/sLac/LuxI III, 2 cultures
  • 19 Pms/sTet/LuxI (on the PCR gel, see above)

File:Gel0509 2.jpg



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