IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/09/06: Difference between revisions
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(Autocreate 2010/09/06 Entry for IGEM:Paris_Liliane_Bettencourt/Notebook/Paris_2010) |
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;">Population counter</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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| colspan="2"| | | colspan="2"| | ||
== | |||
* | <font color=red>Théotime</font> | ||
<br><u>Gel extraction</u> from yesterday | |||
* Pss/wTet/LuxI (9) | |||
* Pss/wTet/LuxI (10) | |||
* Pms/mTet/LuxI (7) | |||
* Pss/sLac/LuxI (17) | |||
* Pms/sLac/LuxI (18) | |||
<br><font color=red>Aleksndra</font> | |||
<br><u>Ligations</u> for cheracterization : same as 03/09, started with the same digestions, burt once again | |||
*sTet+Ps | |||
*mTet+Ps | |||
*wTet+Ps | |||
<br><font color=red>Rphaël</font> | |||
<br><u>Ligations</u> | |||
*pSB1C3 + Pss/wTet/LuxI (9) | |||
*pSB1C3 + Pss/sLac/LuxI (17) | |||
*pSB1C3 + Pms/mTet/LuxI (7) | |||
*pSB1C3 + Pss/wTet/LuxI (10) | |||
*pSB1C3 + Pss/sLac/LuxI (18) | |||
*pSB1C3 ctrl | |||
<br><u>Transformations</u> in TURBO cells | |||
*pSB1C3 + Pss/wTet/LuxI (9) | |||
*pSB1C3 + Pss/sLac/LuxI (17) | |||
*pSB1C3 + Pms/mTet/LuxI (7) | |||
*pSB1C3 + Pss/wTet/LuxI (10) | |||
*pSB1C3 + Pss/sLac/LuxI (18) | |||
*pSB1C3 ctrl | |||
<br><u>Overnight cultures</u> | |||
* Pss/sLac/LuxI III | |||
* LuxI | |||
* RBS/GFP/tt (E0240) | |||
* pLux | |||
* Pss/wTet/LuxI (10), but it didn't grow | |||
* Pms/mTet/LuxI (7) | |||
* Pss/sLac/LuxI III (17), but it didn't grow | |||
* I13507/pLux/GFP | |||
* attP/Pms/attC/RFP, col 1 | |||
* attP/Psm/attC/RFP, col 1 | |||
* attP/Pss/attC/RFP, col 1 | |||
* Pms/mTet/LuxI chr | |||
* Pss/wTet/LuxI chr, but it didn't grow | |||
* pUC chr, but it didn't grow | |||
* PswTet, colonies 1-4 for characterisation | |||
* PsmTet, colonies 1-4 for characterisation | |||
* PssTet, colonies 1-4 for characterisation | |||
<br><u>Touchdown PCR overnight</u> | |||
* PswTet, colonies 1-4 for characterisation | |||
* PsmTet, colonies 1-4 for characterisation | |||
* PssTet, colonies 1-4 for characterisation | |||
<br>But there was a problem with the PCR machine, it didn't work. | |||
Revision as of 09:29, 14 September 2010
 Population counter
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Théotime
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