IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/09/12: Difference between revisions
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<br><u>Glycerols</u> | |||
*Ps-MTet x2 | |||
*Ps-STet x2 | |||
*PsS-WTet-LuxI 1 and 2 | |||
*6h A+ PmS | |||
*5h A+ psS | |||
*6h ctrl A PmS | |||
*5h ctrl A PsS | |||
*5h ctrl A PmS | |||
*4h ctrl A PsS | |||
<br><u>Minipreps</u> | |||
*6h A+ PmS | |||
*5h A+ psS | |||
*6h ctrl A PmS | |||
*5h ctrl A PsS | |||
*5h ctrl A PmS | |||
*4h ctrl A PsS | |||
*PsS-WTet-LuxI 1 and 2 | |||
*Ps-MTet 1 and 2 | |||
*Ps-STet 1 and 2 | |||
<br><u>Gel electrophoresis</u> with PCR from 09/10 | |||
<br>many bands on the gel but the 4Kb band is seen (PmS integrated into the chromosome ?) | |||
<br><u>PCR</u> to verify that the terminator is out in our A+ constructs (primers : VF2 + VR) | |||
*6h A+ PmS | |||
*5h A+ psS | |||
*6h ctrl A PmS | |||
*5h ctrl A PsS | |||
*5h ctrl A PmS | |||
*4h ctrl A PsS | |||
<br><u>Gel electrophoresis</u> | |||
*6h A+ PmS | |||
*5h A+ psS | |||
*6h ctrl A PmS | |||
*5h ctrl A PsS | |||
*5h ctrl A PmS | |||
*4h ctrl A PsS | |||
<br> strange results | |||
<br><u>Ligations</u> (V + I) | |||
* 1) PmS-MTet-LuxI + I13507/pLux/GFP | |||
* 2) pLux/LuxI + RBS-GFP-tt (E0240) | |||
* 3) pLux + LuxI | |||
* 4) Ctrl PmS-MTet-LuxI | |||
* 5) Ctrl pLux/LuxI | |||
* 6) Ctrl pLux | |||
<br><u>Transformation</u> of 1-6 | |||
<br><u>Overnight cultures</u> | |||
*PmS Chr + pBad/Int x2 | |||
Revision as of 08:29, 9 October 2010
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