IGEM:Peking/2007: Difference between revisions

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Interested in being a learning more about iGEM 2007?  Explore below for an example of what's possible.


For more info on this year's iGEM please see [http://www.igem2007.com www.igem2007.com] and the [http://parts.mit.edu/igem07 official iGEM 2007 wiki]
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Many more valuable links and helpful tidbits are available at the [http://parts.mit.edu/wiki/index.php/Resources iGEM Resources page]
'''Hop Count with Counjugation'''
'''Push On Push Off Switch'''


What will be this year's project for the 2007 MIT team?  We're busy brainstorming ideas as you read this!


===External links===
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*[[iGEM|iGEM on OpenWetWare]]
*[http://parts.mit.edu/registry Parts registry]
*[http://parts.mit.edu/igem official iGEM 2006 wiki]
*[http://parts.mit.edu/wiki/index.php/MIT_2006 MIT page on iGEM 2006 wiki]
*Our Parts:
**[http://parts.mit.edu/registry/index.php/Part:BBa_I728005 OmpC Surface Display]
**[http://parts.mit.edu/registry/index.php/Part:BBa_I728004 CPX Surface Display]


===For visitors===
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*Read more about [[Synthetic Biology|engineering biology]].
*[[../2006|2006 MIT iGEM wiki]]
*[[../2005/|2005 MIT iGEM wiki]]
*[[Synthetic_Biology:Polka_Dots|Polka Dots]]: The MIT IAP class, a precursor to iGEM


===Internal links===
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*'''[[../2007/Notebook|Team Notebook]]'''
<img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0">
*'''[[/Red_Team|Red Team]]'''
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*'''[[/Contacts|Contact Numbers]]'''
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*'''[[BE.109:DNA_engineering|DNA engineering from BE.109]]'''
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*'''[[/Protocols|Protocols]]'''
*'''[[/Nomenclature|Nomenclature and Abbreviations]]'''
*'''[[/Codes|Test Tube Codes]]'''
*'''[[/OrderInfo|Order Information]]'''


[[lab calendar]]


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'''Procedures'''
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'''The Protocols'''
<img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources">
</html>Project</h3>
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'''Addressable Cell-to-Cell Communication'''
[[Peking2007-Oligos | Oligonucleotides]]<br>
[[Peking2007-Sequencing | Sequencing]]<br>
[[Peking2007-Miniprep | Miniprep]]<br>
[[Peking2007-Transformation | Transformation]]<br>
[[Peking2007-Digestion | Enzymatic DNA Digestion]]<br>
[[Peking2007-Linking | Linking]]<br>


*[[Media:071906-JCAGroupMeeting.ppt| 071906 JCA Group Meeting]]
'''Notebooks'''
*[[Media:060506-Meeting.ppt| 060506 Meeting]]
*[[Media:Berkeley 2005 Jamboree.ppt| Berkeley 2005 'Jamboree']]
*[http://parts2.mit.edu/wiki/index.php/UC_Berkeley_2005 2005 Project]
*[http://parts.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=iGEM2006&group=iGEM2006_Berkeley '''2006 Parts List''']
'''Useful Links'''


*[[IGEM|iGEM]]
*[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 UC Berkeley iGEM wiki]
*[http://parts2.mit.edu/wiki/index.php/Main_Page iGEM wiki]
*[http://parts.mit.edu/registry/index.php/Main_Page Registry of Standard Biological Parts]
*[http://parts2.mit.edu/r/parts/partsdb/pgroup.cgi?pgroup=iGEM&group=iGEM_Berkeley 2005 Biobricks]
*[[Image:iGEM2006Distribution.xls|iGEM2006 Distribution compiled list]]
*[http://parts2.mit.edu/wiki/index.php/University_of_California_Berkeley_2006 | iGEM site]


<br>


'''Subgroup Strategies, Overview'''
*[[Powerpoint presentations, drafts]]
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Revision as of 10:03, 2 July 2007

Hop count with conjugation

Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. As a prerequisite for hop counting, cell-cell communication should be made on a one to one basis to ensure differential control over neighboring cells. The two possible mechanism of signal transfer are bacterial conjugation and phage. Although a hop count system can be implemented with some modifications to either of the natural systems, we tend to focus on conjugation while doing some basic work on phage. With the conjugation, independent tandem oriT (<bbick) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event. Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems. Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications.

Push-on-push-off Switch

<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Project"> </html>Project


Hop Count with Counjugation Push On Push Off Switch



<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>Lab

lab calendar

Procedures

The Protocols

Oligonucleotides
Sequencing
Miniprep
Transformation
Enzymatic DNA Digestion
Linking

Notebooks




<html> <img src="http://openwetware.org/images/3/39/Icon_groups.png" alt="People"> </html>People

New: iGEM chat room!

Students

Email us: igem-pku [AT] googlegroups.com

Advisors


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