IGEM:Peking/2007

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There are'''<html>
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<div style="font: normal 1.6em georgia, serif">Towards Self-differentiated Bacterial Assembly Line</div>
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Our projects concern with the ability for bacterial cells to '''differentiate''' out of homogeneous conditions into populations with the '''division of labor'''. We aim at devices conferring host cells with the ability to form cooperating groups spontaneously and to take consecutive steps sequentially even when the genetic background and environmental inputs are identical. To break the mirror in such homogeneous condition, we need two devices respectively responsible for '''temporal''' and '''spatial''' differentiation. The implementation and application of such devices will lead to a picture of future bioengineering where complex programs consisted of sequential steps (structure oriented programs) and cooperating agencies (forked instances of a single class, object and event oriented) can be embedded in a single genome.
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Today = new Date();
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Jamboree = new Date("November 3, 2007");
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display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay);
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// </script>
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</html>'''days left until the Jamboree!
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'''Hop Count with Conjugation'''
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<br>
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Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. We decided to use conjugation as our one to one cell-cell communication system and plasmids as the vector for the transfered signal.
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About the name of projects, the two devices are named after their respective functions: hop count and push-on-push-off switch, but they are common for breaking symmetry, which is interesting and ubiquitous in science. Although this "differentiation" process resemble the development of multicellular organism, we tend to use a more bioengineering style analogy: assembly line. Or maybe after some years from now, this will not be just an analogy. <br>
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With the rolling-cycle replication of conjugation, independent tandem oriT (<bbpart>BBa_J01002</bbpart> and more) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event.
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'''Also visit:''' [http://parts.mit.edu/igem07/index.php/Peking '''IGEM2007 Peking''']
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Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems.
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Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications. <br>
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'''Push-on-push-off Switch'''
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We propose to construct a gene regulatory circuit in ''E. coli'' which functions as a push-on/push-off switch. The immediate output of such circuit is determined by the input signal and the internal state of the circuit. It is designed to response to low dose UV irradiation. Under intermittent irradiation, two reporter genes are expected to be induced alternately. 
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{| style="color:#000000; background-color:#EEf8b3;" cellpadding="5" cellspacing="1" border="0" bordercolor="#1100ff" width="820"
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![http://parts.mit.edu/igem07/index.php/Peking IGEM 2007 Peking]
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![http://parts.mit.edu/igem07/index.php/Peking_The_Projects Peking The Projects]
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![http://parts.mit.edu/igem07/index.php/Peking_The_Community Peking The Team]
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![http://parts.mit.edu/igem07/index.php/Peking_The_Fun Peking's Misc & Fun]
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![http://parts.mit.edu/igem07/index.php/Peking_Thanks Acknowledgement]
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[[iGEM:Peking/2007/Count-References | Related Articles ]]<br>
[[iGEM:Peking/2007/Count-References | Related Articles ]]<br>
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'''Push_on_push_off Switch'''<br>
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'''Push-on-push-off Switch'''<br>
[ftp://igem:igem2007@162.105.160.5/OurFiles/SwitchGroup/proposal_switch_revision2.doc Project proposal]<br>
[ftp://igem:igem2007@162.105.160.5/OurFiles/SwitchGroup/proposal_switch_revision2.doc Project proposal]<br>
Project presentation<br>
Project presentation<br>
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Some of our ideas are listed [[iGEM:Peking/2007/Ideas | here]]
Some of our ideas are listed [[iGEM:Peking/2007/Ideas | here]]
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'''Rap up'''<br>
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Collections of the experiences.
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* A [[IGEM:Peking/2007/Experience-colletcion | response]] to Goodman. In English.
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* Collections of [[IGEM:Peking/2007/Things_we_want_to_say |chinese sentiments]].
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[[iGEM:Peking/2007/Switch-Procedure |Push-on-push-off Switch]]<br>
[[iGEM:Peking/2007/Switch-Procedure |Push-on-push-off Switch]]<br>
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'''Protocols'''
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'''Protocols'''<br>
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[[iGEM:Peking/2007/Count:Agarose Gel Electrophoresis| Count:Agarose Gel Electrophoresis]]<br>
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[[iGEM:Peking/2007/Count:Gel-Extraction | Count:DNA Gel Extraction]]<br>
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[[iGEM:Peking/2007/Oligos | Oligonucleotides]]<br>
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[[iGEM:Peking/2007/Count:PCR product purifcation| Count:PCR product purifcation]]<br>
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[[iGEM:Peking/2007/Count:Agarose Gel Electrophoresis| Agarose Gel Electrophoresis]]<br>
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[[iGEM:Peking/2007/Count:Preparation of Competent Cells | Count:Preparation of Competent Cells]]<br>
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[[iGEM:Peking/2007/Count:Gel-Extraction | DNA Gel Extraction]]<br>
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[[iGEM:Peking/2007/Count:PCR product purifcation| PCR product purifcation]]<br>
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[[iGEM:Peking/2007/Count:Preparation of Competent Cells | Preparation of Competent Cells]]<br>
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[[iGEM:Peking/2007/Digestion | Enzymatic DNA Digestion]]<br>
[[iGEM:Peking/2007/Digestion | Enzymatic DNA Digestion]]<br>
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[[iGEM:Peking/2007/Ligation | Ligation]]<br>
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[[iGEM:Peking/2007/Count:Ligation | Count:Ligation]]<br>
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[[iGEM:Peking/2007/Transformation | Transformation]]<br>
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[[iGEM:Peking/2007/Count:Transformation | Count:Transformation]]<br>
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[[iGEM:Peking/2007/Sequencing | Sequencing]]<br>
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[[iGEM:Peking/2007/Count:Sequencing | Count:Sequencing]]<br>
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[[iGEM:Peking/2007/Switch:Miller | Switch: Miller Method]]<br>
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[[iGEM:Peking/2007/Count:Miniprep | Count: Miniprep]]<br>
[[iGEM:Peking/2007/Count:Miniprep | Count: Miniprep]]<br>
[[iGEM:Peking/2007/Count:Conjugation| Count: Conjugation test]]<br>
[[iGEM:Peking/2007/Count:Conjugation| Count: Conjugation test]]<br>
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[[iGEM:Peking/2007/Count:Knockout| Count: Gene Knock Out]]<br>
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[[iGEM:Peking/2007/Switch:Miller | Switch: Miller Method]]<br>
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[[iGEM:Peking/2007/Oligos | Oligonucleotides]]<br>
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[[iGEM:Peking/2007/Switch: PCR | Switch: PCR]]<br>
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[[iGEM:Peking/2007/Switch: DNA electrophoresis | Switch: DNA electrophoresis]]<br>
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[[iGEM:Peking/2007/Switch: DNA ligation | Switch: DNA ligation]]<br>
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[[iGEM:Peking/2007/Switch: DNA digestion | Switch: DNA digestion]]<br>
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[[iGEM:Peking/2007/Switch: transformation | Switch: transformation]]<br>
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[[iGEM:Peking/2007/Switch: DNA rescue by precipitation | Switch: DNA rescue by precipitation]]<br>
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[[iGEM:Peking/2007/Switch: Plasmid mini prep| Switch: Plasmid mini prep]]<br>
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'''Notebooks'''<br>
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[[Image:Peking_2007_Notebook_icon.jpg‎|20px]]'''Notebooks'''<br>
[[iGEM:Peking/2007/Count-Conjugation-Notebook |Hop-count with Conjugation]]<br>
[[iGEM:Peking/2007/Count-Conjugation-Notebook |Hop-count with Conjugation]]<br>
[[iGEM:Peking/2007/Switch_Notebook|Push-on-push-off Switch]]<br>
[[iGEM:Peking/2007/Switch_Notebook|Push-on-push-off Switch]]<br>
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=== Students ===
=== Students ===
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Please sign up [[iGEM:Peking/2007/Student-Members|here]].
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*[[iGEM:Peking/2007/Anting Xu | Anting Xu]]
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*[[iGEM:Peking/2007/Chang Liu | Chang Liu]]
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*[[iGEM:Peking/2007/Chongyi Chen | Chongyi Chen]]
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*[[iGEM:Peking/2007/Chunbo Lou | Chunbo Lou]]
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*[[User:Chen Daizhuo | Chen Daizhuo, Henry]]
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*[[iGEM:Peking/2007/Dan Lu | Dan Lu]]
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*[[iGEM:Peking/2007/Haisu Ma | Haisu Ma]]
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*[[iGEM:Peking/2007/Lingli Jiang | Lingli Jiang]]
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*[[iGEM:Peking/2007/Ming Ni | Ming Ni]]
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*[[iGEM:Peking/2007/Mingcong Wang | Mingcong Wang]]
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*[[Peking_Mingzhi Qu | Mingzhi Qu]]
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*[[iGEM:Peking/2007/Qinsi Zheng | Qinsi Zheng]]
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*[[iGEM:Peking/2007/Tao Ma | Tao Ma]]
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*[[iGEM:Peking/2007/Tao Yu | Tao Yu ]]
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*[[iGEM:Peking/2007/Ting Liu | Ting Liu]]
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*[[iGEM:Peking/2007/Xili Liu | Xili Liu]]
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*[[User:YangYifan | Yifan Yang]]
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*[[iGEM:Peking/2007/Zhe Ren | Zhe Ren]]
=== Advisors ===
=== Advisors ===
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'''Email us''' at ''igem-pku AT googlegroups DOT com''
'''Email us''' at ''igem-pku AT googlegroups DOT com''
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'''External links''' [http://parts.mit.edu/wiki/index.php/Main_Page iGEM] | [http://www.igem2006.com iGEM 2006] | [http://parts.mit.edu/ Registry of Standard Biological Parts] | [http://groups.google.com/group/igem-beijing/topics?lnk=oa iGEM PKU Googlegroup] | [[iGEM|iGEM OWW Page]]
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'''External links''' [http://parts.mit.edu/wiki/index.php/Main_Page iGEM] | [http://www.igem2006.com iGEM 2006] | [http://parts.mit.edu/ Registry of Standard Biological Parts] | [http://groups.google.com/group/igem-pku/topics?lnk=oa iGEM PKU Googlegroup] | [[iGEM|iGEM OWW Page]]

Current revision



Towards Self-differentiated Bacterial Assembly Line

Our projects concern with the ability for bacterial cells to differentiate out of homogeneous conditions into populations with the division of labor. We aim at devices conferring host cells with the ability to form cooperating groups spontaneously and to take consecutive steps sequentially even when the genetic background and environmental inputs are identical. To break the mirror in such homogeneous condition, we need two devices respectively responsible for temporal and spatial differentiation. The implementation and application of such devices will lead to a picture of future bioengineering where complex programs consisted of sequential steps (structure oriented programs) and cooperating agencies (forked instances of a single class, object and event oriented) can be embedded in a single genome.


About the name of projects, the two devices are named after their respective functions: hop count and push-on-push-off switch, but they are common for breaking symmetry, which is interesting and ubiquitous in science. Although this "differentiation" process resemble the development of multicellular organism, we tend to use a more bioengineering style analogy: assembly line. Or maybe after some years from now, this will not be just an analogy.
Also visit: IGEM2007 Peking


IGEM 2007 Peking Peking The Projects Peking The Team Peking's Misc & Fun Acknowledgement

Project Project

Hop Count with Conjugation
Project Proposal
Project Presentation
Road Map
Related Articles

Push-on-push-off Switch
Project proposal
Project presentation
Road Map
Related Articles

Meetings

Brainstorming
Some of our ideas are listed here

Rap up
Collections of the experiences.

News Lab

Products and Parts

Procedures
Hop-count with Conjugation
Push-on-push-off Switch

Protocols
Count:Agarose Gel Electrophoresis
Count:DNA Gel Extraction
Count:PCR product purifcation
Count:Preparation of Competent Cells
Enzymatic DNA Digestion
Count:Ligation
Count:Transformation
Count:Sequencing
Count: Miniprep
Count: Conjugation test
Count: Gene Knock Out
Switch: Miller Method
Oligonucleotides
Switch: PCR
Switch: DNA electrophoresis
Switch: DNA ligation
Switch: DNA digestion
Switch: transformation
Switch: DNA rescue by precipitation
Switch: Plasmid mini prep



Notebooks
Hop-count with Conjugation
Push-on-push-off Switch
Turtle Diary



People People

The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology.

Students

Advisors

Photo

The Team

Our Campus

The Labs

Email us at igem-pku AT googlegroups DOT com

External links iGEM | iGEM 2006 | Registry of Standard Biological Parts | iGEM PKU Googlegroup | iGEM OWW Page

Personal tools