IGEM:Peking/2007

From OpenWetWare
Revision as of 11:09, 2 July 2007 by YangYifan (talk | contribs)
Jump to navigationJump to search

Hop count with conjugation

Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. We decided to use conjugation as our one to one cell-cell communication system and plasmids as the vector for the transfered signal. With the rolling-cycle replication of conjugation, independent tandem oriT (<bbpart>BBa_J01002</bbpart> and more) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event. Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems. Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications.

Push-on-push-off Switch


<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Project"> </html>Project



Hop Count with Conjugation
Project Proposal
Project Presentation
Road Map
Related Articles

Push On Push Off Switch
Project proposal
Project presentation
Road Map
Related Articles

Meetings

Brainstorming

<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>Lab


Procedures

Protocols

Oligonucleotides
Sequencing
Miniprep
Transformation
Enzymatic DNA Digestion
Linking

Notebooks




<html> <img src="http://openwetware.org/images/3/39/Icon_groups.png" alt="People"> </html>People


The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology.

Students

Please sign up here.

Advisors

Photo

The Team

Our Campus

The Labs

Email us at igem-pku AT googlegroups DOT com

External links iGEM | iGEM 2006 | Registry of Standard Biological Parts | iGEM PKU Googlegroup