IGEM:Peking/2007

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There are days left until the Jamboree!

Hop Count with Conjugation Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. We decided to use conjugation as our one to one cell-cell communication system and plasmids as the vector for the transfered signal. With the rolling-cycle replication of conjugation, independent tandem oriT (<bbpart>BBa_J01002</bbpart> and more) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event. Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems. Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications.
Push-on-push-off Switch We propose to construct a gene regulatory circuit in E. coli which functions as a push-on/push-off switch. The immediate output of such circuit is determined by the input signal and the internal state of the circuit. It is designed to response to low dose UV irradiation. Under intermittent irradiation, two reporter genes are expected to be induced alternately.


Project Project

Hop Count with Conjugation
Project Proposal
Project Presentation
Road Map
Related Articles

Push_on_push_off Switch
Project proposal
Project presentation
Road Map
Related Articles

Meetings

Brainstorming
Some of our ideas are listed here


News Lab

Products and Parts

Procedures
Hop-count with Conjugation
Push-on-push-off Switch

Protocols


Oligonucleotides
Agarose Gel Electrophoresis
DNA Gel Extraction
PCR product purifcation
Preparation of Competent Cells
Enzymatic DNA Digestion
Ligation
Transformation
Sequencing
Switch: Miller Method
Count: Miniprep
Count: Conjugation test



Notebooks
Hop-count with Conjugation
Push-on-push-off Switch
Turtle Diary



People People

The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology.

Students

Please sign up here.

Advisors

Photo

The Team

Our Campus

The Labs

Email us at igem-pku AT googlegroups DOT com

External links iGEM | iGEM 2006 | Registry of Standard Biological Parts | iGEM PKU Googlegroup | iGEM OWW Page

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