IGEM:Peking/2007

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There are days left until the Jamboree!

Hop Count with Conjugation Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. We decided to use conjugation as our one to one cell-cell communication system and plasmids as the vector for the transfered signal. With the rolling-cycle replication of conjugation, independent tandem oriT (<bbpart>BBa_J01002</bbpart> and more) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event. Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems. Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications.
Push-on-push-off Switch We propose to construct a gene regulatory circuit in E. coli which functions as a push-on/push-off switch. The immediate output of such circuit is determined by the input signal and the internal state of the circuit. It is designed to response to low dose UV irradiation. Under intermittent irradiation, two reporter genes are expected to be induced alternately.
visit also:| IGEM2007 Peking


Project Project

Hop Count with Conjugation
Project Proposal
Project Presentation
Road Map
Related Articles

Push-on-push-off Switch
Project proposal
Project presentation
Road Map
Related Articles

Meetings

Brainstorming
Some of our ideas are listed here


News Lab

Products and Parts

Procedures
Hop-count with Conjugation
Push-on-push-off Switch

Protocols
Count:Agarose Gel Electrophoresis
Count:DNA Gel Extraction
Count:PCR product purifcation
Count:Preparation of Competent Cells
Enzymatic DNA Digestion
Count:Ligation
Count:Transformation
Count:Sequencing
Count: Miniprep
Count: Conjugation test
Count: Gene Knock Out
Switch: Miller Method
Oligonucleotides
Switch: PCR
Switch: DNA electrophoresis
Switch: DNA ligation
Switch: DNA digestion
Switch: transformation
Switch: DNA rescue by precipitation
Switch: Plasmid mini prep



Notebooks
Hop-count with Conjugation
Push-on-push-off Switch
Turtle Diary



People People

The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology.

Students

  • Anting Xu
  • Chang Liu
  • Chongyi Chen
  • Chunbo Lou
  • Daizhuo Chen
  • Dan Lu
  • Haisu Ma
  • Lingli Jiang
  • Ming Ni
  • Mingcong Wang
  • Mingzhi Qu
  • Qinsi Zheng
  • Tao Ma
  • Tao Yu
  • Ting Liu
  • Xili Liu
  • Yifan Yang
  • Zhe Ren

Please sign up here.

Advisors

Photo

The Team

Our Campus

The Labs

Email us at igem-pku AT googlegroups DOT com

External links iGEM | iGEM 2006 | Registry of Standard Biological Parts | iGEM PKU Googlegroup | iGEM OWW Page

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