IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-10: Difference between revisions
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==Another mistake when transforming (0：10:10) == | ==Start up== | ||
===Another mistake when transforming (0：10:10) === | |||
The following experiment takes place between 0:30 to 0:50<br> | The following experiment takes place between 0:30 to 0:50<br> | ||
Qu:Should i centrifuge these tubes ?<br> | Qu:Should i centrifuge these tubes ?<br> | ||
| Line 17: | Line 18: | ||
Qu&Ma&Zheng&yu:...........what the ****(di~)...<br> | Qu&Ma&Zheng&yu:...........what the ****(di~)...<br> | ||
==Bacteria cultivation from CGMCC== | ===Bacteria cultivation from CGMCC=== | ||
K-12 F+: nutrient bouillon agar with 30℃ | * By Xu Anting and Liu Ting | ||
:K-12 F+: nutrient bouillon agar with 30℃ | |||
:Wait to see if the protocol from CGMCC (China General Microbiological Culture Collection Centre) is OK... | |||
===Transformation plasmid from Biobricks=== | |||
#Transformation Biobrick PARTS：pSB1A3, pSB3K3, pSB1AK3, R0010, J23066. | |||
Latest revision as of 07:50, 10 July 2007
Start up
Another mistake when transforming (0：10:10)
The following experiment takes place between 0:30 to 0:50
Qu:Should i centrifuge these tubes ?
Zheng:Just do it.
Qu: Roger that(centrifuging)...
after 5 minutes....(centrifuge finished)
Yu:where's the tubes....
Qu:I've just Finished centrifuging ...
Yu:these tubes must be standing in ice for 30 minutes before taking them to ....
Qu&Ma&Zheng&Yu: ..........
after 10 minutes....
Yu:We can use these tubes for Nagetive control!
Zheng:Yep.
Ma:GO GO GO...
5 minutes later...(centrifuge finished)
Yu:I forgot to mark nagetive control tubes
Qu&Ma&Zheng&yu:...........what the ****(di~)...
Bacteria cultivation from CGMCC
- By Xu Anting and Liu Ting
- K-12 F+: nutrient bouillon agar with 30℃
- Wait to see if the protocol from CGMCC (China General Microbiological Culture Collection Centre) is OK...
Transformation plasmid from Biobricks
- Transformation Biobrick PARTS：pSB1A3, pSB3K3, pSB1AK3, R0010, J23066.
