IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-13

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Revision as of 06:40, 13 July 2007 by Anting Xu (talk | contribs) (New page: ===First Test of oriT Knock Out Primers=== *By Xu Anting **Colony PCR. Temperature gradient is 45℃ to 65℃, with a volume of 10 uL ::Result: F primers have bands at 51℃, but R751 and ...)
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First Test of oriT Knock Out Primers

  • By Xu Anting
    • Colony PCR. Temperature gradient is 45℃ to 65℃, with a volume of 10 uL
Result: F primers have bands at 51℃, but R751 and pSC101 show negative results in two separate tests.
    • Next task: Use plasmids as template to do PCR test.
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