IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-18: Difference between revisions
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Qu Mingzhi (talk | contribs) (New page: ===Miniprep=== *Miniprep & preservation:I7100. ===Digestion Test=== *For I7100 and E0040. *EcoR I single digestion. *Our digestion system contains (totally 20uL): 2uL 10*H, 0.5uL EcoR I, ...) |
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*EcoR I single digestion. | *EcoR I single digestion. | ||
*Our digestion system contains (totally 20uL): 2uL 10*H, 0.5uL EcoR I, 3uL plasmid, 15uL ddH2O. | *Our digestion system contains (totally 20uL): 2uL 10*H, 0.5uL EcoR I, 3uL plasmid, 15uL ddH2O. | ||
*from ''Left'' to ''Right'': | |||
#I7100 @ EcoRI | |||
#E0040 @ EcoRI | |||
#I7100 plasmid | |||
#E0040 plasmid | |||
#Marker | |||
[[Image:2007-7-18_I7100_E0040_EcoRI.jpg ]] | |||
===Double Digesting of E0040 and I7100 for ligation and electrophoresis=== | |||
Revision as of 03:26, 30 July 2007
Miniprep
- Miniprep & preservation:I7100.
Digestion Test
- For I7100 and E0040.
- EcoR I single digestion.
- Our digestion system contains (totally 20uL): 2uL 10*H, 0.5uL EcoR I, 3uL plasmid, 15uL ddH2O.
- from Left to Right:
- I7100 @ EcoRI
- E0040 @ EcoRI
- I7100 plasmid
- E0040 plasmid
- Marker
