IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-18: Difference between revisions
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==Miniprep== | |||
*Miniprep & preservation:I7100. | *Miniprep & preservation:I7100. | ||
==Digestion Test== | |||
*For I7100 and E0040. | *For I7100 and E0040. | ||
*EcoR I single digestion. | *EcoR I single digestion. | ||
| Line 14: | Line 14: | ||
[[Image:2007-7-18_I7100_E0040_EcoRI.jpg ]] | [[Image:2007-7-18_I7100_E0040_EcoRI.jpg ]] | ||
==Double Digesting of E0040 and I7100 for ligation and electrophoresis== | |||
*Digesting E0040 and I7100 with EcoR1/SpeI and EcoRI/XbaI. | *Digesting E0040 and I7100 with EcoR1/SpeI and EcoRI/XbaI. | ||
*Digestion system contains (totally 20uL): | *Digestion system contains (totally 20uL): | ||
Revision as of 03:41, 30 July 2007
Miniprep
- Miniprep & preservation:I7100.
Digestion Test
- For I7100 and E0040.
- EcoR I single digestion.
- Digestion system contains (totally 20uL): 2uL 10*H, 0.5uL EcoR I, 3uL plasmid, 15uL ddH2O.
- from Left to Right:
- I7100 @ EcoRI
- E0040 @ EcoRI
- I7100 plasmid
- E0040 plasmid
- Marker
Double Digesting of E0040 and I7100 for ligation and electrophoresis
- Digesting E0040 and I7100 with EcoR1/SpeI and EcoRI/XbaI.
- Digestion system contains (totally 20uL):
- 3uL 10*H, 1uL EcoRI, 1uL SpeI, 6uL plasmid, 10uL ddH2O.
- 3uL 10*M, 1uL EcoRI, 1uL XbaI, 6uL plasmid, 10uL ddH2O.
- 37℃ culutre for 12 hours.
- from Left to Right:
