IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-18

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Miniprep

  • Miniprep & preservation:I7100.

Digestion Test

  • For I7100 and E0040.
  • EcoR I single digestion.
  • Our digestion system contains (totally 20uL): 2uL 10*H, 0.5uL EcoR I, 3uL plasmid, 15uL ddH2O.
  • from Left to Right:
  1. I7100 @ EcoRI
  2. E0040 @ EcoRI
  3. I7100 plasmid
  4. E0040 plasmid
  5. Marker

Double Digesting of E0040 and I7100 for ligation and electrophoresis

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