IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-20: Difference between revisions
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*! there are abnormal bands in the e0040 samples. | *! there are abnormal bands in the e0040 samples. | ||
[[Image:E0040-3-4-5_EcoR1 and R0040-2-4_EcoR1.jpg]] | |||
==another single digestion test== | ==another single digestion test== |
Revision as of 23:35, 23 July 2007
double digesting e0040 and I7100 for ligation and electrophoresis
- digesting e0040 and I7100 with EcoR1 and Pst1, restectively
- for about 12 hours
- from left to right:
1 I7100 plasmid
2-5 I7100 and EcoR1 and Pst1
6 e0040 plasmid
7-10 e0040 and EcoR1 and Pst1
11 marker DL200 plus
Gel Extraction
- extract pSB3k3(I7100) vector and e0040 fragments from gel
electrophoresis test the products of gel extraction
- from left to right:
EGFP(e0040)
pSB3k3(I7100)
Marker DL200 plus
ligation
- ligate the pSB3k3(I7100) and EGFP(E0040) with T4 ligase
mini-prep I7100 and E0040
single digestion test and electrophoresis
- digest e0040 and r0040 with EcoR1, respectively
- for about 3 hours
- from left to right:
1-3 e0040 and EcoR1
4-5 r0040 and EcoR1
6 Marker
- ! there are abnormal bands in the e0040 samples.
another single digestion test
- digest I7100 with Xba1
- digest e0040(another sample) with EcoR1
- electrophoresis results. From left to right:
1 I7100 plasmid
2-4 I7100 and Xba1
5 e0040 plasmid
6-8 e0040 and EcoR1
9 marker DL2000 plus
10 r0040 and EcoR1 (products of former digestion test)