IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-20

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(another single digestion test)
(single digestion test and electrophoresis)
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*! there are abnormal bands in the e0040 samples.
*! there are abnormal bands in the e0040 samples.
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[[Image:E0040-3-4-5_EcoR1 and R0040-2-4_EcoR1.jpg]]
==another single digestion test==
==another single digestion test==

Revision as of 01:35, 24 July 2007

Contents

double digesting e0040 and I7100 for ligation and electrophoresis

  • digesting e0040 and I7100 with EcoR1 and Pst1, restectively
  • for about 12 hours
  • from left to right:

1 I7100 plasmid

2-5 I7100 and EcoR1 and Pst1

6 e0040 plasmid

7-10 e0040 and EcoR1 and Pst1

11 marker DL200 plus

Gel Extraction

  • extract pSB3k3(I7100) vector and e0040 fragments from gel

electrophoresis test the products of gel extraction

  • from left to right:

EGFP(e0040)

pSB3k3(I7100)

Marker DL200 plus

ligation

  • ligate the pSB3k3(I7100) and EGFP(E0040) with T4 ligase



mini-prep I7100 and E0040

single digestion test and electrophoresis

  • digest e0040 and r0040 with EcoR1, respectively
  • for about 3 hours
  • from left to right:

1-3 e0040 and EcoR1

4-5 r0040 and EcoR1

6 Marker

  • ! there are abnormal bands in the e0040 samples.

Image:E0040-3-4-5_EcoR1 and R0040-2-4_EcoR1.jpg

another single digestion test

  • digest I7100 with Xba1
  • digest e0040(another sample) with EcoR1
  • electrophoresis results. From left to right:

1 I7100 plasmid

2-4 I7100 and Xba1

5 e0040 plasmid

6-8 e0040 and EcoR1

9 marker DL2000 plus

10 r0040 and EcoR1 (products of former digestion test)

Image:I7100-3-4-5 XbaI and E0040-6-7-8 EcoRI and R0040-4 EcoRI.jpg

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