IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-22: Difference between revisions
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Qinsi Zheng (talk | contribs) |
Qinsi Zheng (talk | contribs) |
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===mini-prep e0040->pSB3k3=== | ===mini-prep e0040->pSB3k3=== | ||
===double digesting test of e0040->pSB3k3=== | ===double digesting test of e0040->pSB3k3 and electrophorsis=== | ||
*from left to right: | *from left to right: | ||
*1 e0040->pSB3k3-1 and EcoR1 and Pst1 | *1 e0040->pSB3k3-1 and EcoR1 and Pst1 | ||
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[[Image:2007-7-22Ligation(EGFP+pSB3k3)-1_EcoR1_Pst1_and_ligation(EGFP+pSB3k3)-2_EcoR1_Pst1.jpg]] | [[Image:2007-7-22Ligation(EGFP+pSB3k3)-1_EcoR1_Pst1_and_ligation(EGFP+pSB3k3)-2_EcoR1_Pst1.jpg]] | ||
* I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow. | |||
Revision as of 20:21, 23 July 2007
mini-prep e0040->pSB3k3
double digesting test of e0040->pSB3k3 and electrophorsis
- from left to right:
- 1 e0040->pSB3k3-1 and EcoR1 and Pst1
- 2 e0040->pSB3k3-2 and EcoR1 and Pst1
- 3 control:e0040 and EcoR1 and Pst1
- 4 I7100 and EcoR1 and Pst1
- 5 e0040->pSB3k3-1 plasmid
- 6 e0040->pSB3k3-2 plasmid
- 7 Marker: DL2000 plus
-1_EcoR1_Pst1_and_ligation(EGFP%2BpSB3k3)-2_EcoR1_Pst1.jpg)
- I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.
