IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-22: Difference between revisions

Latest revision as of 08:57, 30 July 2007

I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.

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-===mini-prep e0040->pSB3k3===
+==mini-prep e0040->pSB3k3==
-===double digesting test of e0040->pSB3k3===
+==double digesting test of E0040->pSB3k3==
-*from left to right:
+*digesting (GFP+pSB3K3) and E0040 with EcoR1/PstI.
-e0040->pSB3k3-1 and EcoR1 and Pst1
+*Digestion system contains :
-e0040->pSB3k3-2 and EcoR1 and Pst1
+#(GFP+pSB3K3): 2uL 10*H, 0.5uL EcoRI, 0.5uL PstI, 5uL plasmid, 13uL ddH2O.
-control:e0040 and EcoR1 and Pst1
+#E0040:2uL 10*H, 0.5uL EcoRI, 0.5uL PstI, 4uL plasmid, 13uL ddH2O.
-I7100 and EcoR1 and Pst1
+*37℃ culutre for 2 hours.
-e0040->pSB3k3-1 plasmid
-e0040->pSB3k3-2 plasmid
+===electrophorsis result===
-Marker: DL2000 plus
+*from ''left'' to ''right'':
+# e0040->pSB3k3-1 @ EcoRI/Pst1
+# e0040->pSB3k3-2 @ EcoRI/Pst1
+# control:e0040 @ EcoRI/Pst1
+# I7100 @ EcoRI/Pst1
+# e0040->pSB3k3-1 plasmid
+# e0040->pSB3k3-2 plasmid
+# Marker: DL2000 plus
+[[Image:2007-7-22Ligation(EGFP+pSB3k3)-1_EcoR1_Pst1_and_ligation(EGFP+pSB3k3)-2_EcoR1_Pst1.jpg]]
+* I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.