IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-22: Difference between revisions

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==mini-prep e0040->pSB3k3==
==mini-prep e0040->pSB3k3==


==double digesting test of e0040->pSB3k3 and electrophorsis==
==double digesting test of E0040->pSB3k3==
*dgesting E0040 and I7100 with EcoR1/SpeI.
*digesting (GFP+pSB3K3) and E0040 with EcoR1/PstI.
*Digestion system contains :
#(GFP+pSB3K3): 2uL 10*H, 0.5uL EcoRI, 0.5uL PstI, 5uL plasmid, 13uL ddH2O.
#E0040:2uL 10*H, 0.5uL EcoRI, 0.5uL PstI, 4uL plasmid, 13uL ddH2O.
*37℃ culutre for 2 hours.  
 
===electrophorsis result===
*from ''left'' to ''right'':
*from ''left'' to ''right'':
*1 e0040->pSB3k3-1 @ EcoRI/Pst1  
# e0040->pSB3k3-1 @ EcoRI/Pst1  
*2 e0040->pSB3k3-2 @ EcoRI/Pst1  
# e0040->pSB3k3-2 @ EcoRI/Pst1  
*3 control:e0040 @ EcoRI/Pst1
# control:e0040 @ EcoRI/Pst1
*4 I7100 @ EcoRI/Pst1  
# I7100 @ EcoRI/Pst1  
*5 e0040->pSB3k3-1 plasmid  
# e0040->pSB3k3-1 plasmid  
*6 e0040->pSB3k3-2 plasmid  
# e0040->pSB3k3-2 plasmid  
*7 Marker: DL2000 plus
# Marker: DL2000 plus


[[Image:2007-7-22Ligation(EGFP+pSB3k3)-1_EcoR1_Pst1_and_ligation(EGFP+pSB3k3)-2_EcoR1_Pst1.jpg]]
[[Image:2007-7-22Ligation(EGFP+pSB3k3)-1_EcoR1_Pst1_and_ligation(EGFP+pSB3k3)-2_EcoR1_Pst1.jpg]]


* I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.
* I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.

Latest revision as of 08:57, 30 July 2007

mini-prep e0040->pSB3k3

double digesting test of E0040->pSB3k3

  • digesting (GFP+pSB3K3) and E0040 with EcoR1/PstI.
  • Digestion system contains :
  1. (GFP+pSB3K3): 2uL 10*H, 0.5uL EcoRI, 0.5uL PstI, 5uL plasmid, 13uL ddH2O.
  2. E0040:2uL 10*H, 0.5uL EcoRI, 0.5uL PstI, 4uL plasmid, 13uL ddH2O.
  • 37℃ culutre for 2 hours.

electrophorsis result

  • from left to right:
  1. e0040->pSB3k3-1 @ EcoRI/Pst1
  2. e0040->pSB3k3-2 @ EcoRI/Pst1
  3. control:e0040 @ EcoRI/Pst1
  4. I7100 @ EcoRI/Pst1
  5. e0040->pSB3k3-1 plasmid
  6. e0040->pSB3k3-2 plasmid
  7. Marker: DL2000 plus

  • I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.
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