IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-22

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(double digesting test of e0040->pSB3k3)
(double digesting test of e0040->pSB3k3)
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===mini-prep e0040->pSB3k3===
===mini-prep e0040->pSB3k3===
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===double digesting test of e0040->pSB3k3===
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===double digesting test of e0040->pSB3k3 and electrophorsis===
*from left to right:
*from left to right:
*1 e0040->pSB3k3-1 and EcoR1 and Pst1  
*1 e0040->pSB3k3-1 and EcoR1 and Pst1  
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[[Image:2007-7-22Ligation(EGFP+pSB3k3)-1_EcoR1_Pst1_and_ligation(EGFP+pSB3k3)-2_EcoR1_Pst1.jpg]]
[[Image:2007-7-22Ligation(EGFP+pSB3k3)-1_EcoR1_Pst1_and_ligation(EGFP+pSB3k3)-2_EcoR1_Pst1.jpg]]
 +
 +
* I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.

Revision as of 23:21, 23 July 2007

mini-prep e0040->pSB3k3

double digesting test of e0040->pSB3k3 and electrophorsis

  • from left to right:
  • 1 e0040->pSB3k3-1 and EcoR1 and Pst1
  • 2 e0040->pSB3k3-2 and EcoR1 and Pst1
  • 3 control:e0040 and EcoR1 and Pst1
  • 4 I7100 and EcoR1 and Pst1
  • 5 e0040->pSB3k3-1 plasmid
  • 6 e0040->pSB3k3-2 plasmid
  • 7 Marker: DL2000 plus

Image:2007-7-22Ligation(EGFP+pSB3k3)-1_EcoR1_Pst1_and_ligation(EGFP+pSB3k3)-2_EcoR1_Pst1.jpg

  • I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.
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