IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-22

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Revision as of 08:51, 30 July 2007 by Qu Mingzhi (talk | contribs)
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mini-prep e0040->pSB3k3

double digesting test of e0040->pSB3k3 and electrophorsis

  • dgesting E0040 and I7100 with EcoR1/SpeI.
  • from left to right:
  • 1 e0040->pSB3k3-1 @ EcoRI/Pst1
  • 2 e0040->pSB3k3-2 @ EcoRI/Pst1
  • 3 control:e0040 @ EcoRI/Pst1
  • 4 I7100 @ EcoRI/Pst1
  • 5 e0040->pSB3k3-1 plasmid
  • 6 e0040->pSB3k3-2 plasmid
  • 7 Marker: DL2000 plus

  • I think there may be mistakes while electrophorsising. Re-electrophorsis tommorrow.