IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-23: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: Digested crRNA ( J23078 ) and R0040 for ligation tommorrow.) |
No edit summary |
||
| Line 1: | Line 1: | ||
Digested crRNA ( J23078 ) and R0040 for ligation tommorrow. | Digested crRNA ( J23078 ) and R0040 for ligation tommorrow. | ||
===oriT Knock Out=== | |||
*By Xu Anting | |||
:Use chromosome extraction product as template to do PCR, with Taq or Pfu added. | |||
::Result: In Taq PCR all 6 pairs of primers show bright bands at 500 bp, while none is shown in Pfu PCR. | |||
::Conclusion: finally I got oriT up- and downstream fragments of 500 bp with potential site mutations caused by Taq's relatively low fidelity. I intend to use these products as template to do further PCR and get oriT-deleted fragments of 1200 bp. | |||
Revision as of 07:23, 23 July 2007
Digested crRNA ( J23078 ) and R0040 for ligation tommorrow.
oriT Knock Out
- By Xu Anting
- Use chromosome extraction product as template to do PCR, with Taq or Pfu added.
- Result: In Taq PCR all 6 pairs of primers show bright bands at 500 bp, while none is shown in Pfu PCR.
- Conclusion: finally I got oriT up- and downstream fragments of 500 bp with potential site mutations caused by Taq's relatively low fidelity. I intend to use these products as template to do further PCR and get oriT-deleted fragments of 1200 bp.
