IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-23

Riboregulator

Digested crRNA ( J23078 ) and R0040 for ligation tommorrow.

oriT Knock Out

• By Xu Anting

Use chromosome extraction product as template to do PCR, with Taq or Pfu added.

Result: In Taq PCR all 6 pairs of primers show bright bands at 500 bp, while none is shown in Pfu PCR.

Conclusion: finally I got oriT up- and downstream fragments of 500 bp with potential site mutations caused by Taq's relatively low fidelity. I intend to use these products as template to do further PCR and get oriT-deleted fragments of 1200 bp.