IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-30

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Lock & Key By Yu Tao

oriT Knock Out

  • By Xu Anting

Competent Cell preparation

  • Prepare to make competent cells using strain F/R751/pSC101
  • Sterilize 1M CaCl2, 100mM CaCl2/15% glycerol.
  • Activation of three strains in LB- plate (pSC101 in Tc+ plate). Hold in 37 centigrade for 16 hours.
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