IGEM:Peking/2007/Count-Conjugation-Notebook/2007-7-9: Difference between revisions
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actagagtgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggagatactag | actagagtgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggagatactag | ||
* We order two primer overlapping by 13 bp:<br> | * We order two primer overlapping by 13 bp:<br> | ||
>igempku-crRNA-J23078-primer1 | >igempku-crRNA-J23078-primer1<br> | ||
cggccgcttctagagtgtagaccgaactagaatcacctcttgcttttggg | cggccgcttctagagtgtagaccgaactagaatcacctcttgcttttggg<br> | ||
>igempku-crRNA-J23078-primer2 | >igempku-crRNA-J23078-primer2<br> | ||
actagtatctcctcttctgtcttacccaaaagcaagaggtgattctagt | actagtatctcctcttctgtcttacccaaaagcaagaggtgattctagt<br> | ||
==E.coli strains fetched from CGMCC== | ==E.coli strains fetched from CGMCC== | ||
Revision as of 01:28, 9 July 2007
Order the <bbpart>pSB1A2</bbpart> sequencing primer
- vf2: tgccacctgacgtctaagaa
- vr: attaccgcctttgagtgag
Order the BBa_J23078 synthesizing primer
- We want to synthesize J23078 with a NotI-XbaI prefix and SpeI suffix:
cggccgcttctagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcact actagagtgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggagatactag
- We order two primer overlapping by 13 bp:
>igempku-crRNA-J23078-primer1
cggccgcttctagagtgtagaccgaactagaatcacctcttgcttttggg
>igempku-crRNA-J23078-primer2
actagtatctcctcttctgtcttacccaaaagcaagaggtgattctagt
E.coli strains fetched from CGMCC
- 3 strains containing F, R751, pSC101 plasmids
- CGMCC accession: 1.1137 -> pSC101; 1.1501 -> R751; 1.747 -〉F
- Culture media included
- fetched by Yu Tao
Knockout primer design
- Xu Anting and Ma Tao
- Read:;
- Lanka, E. & Wilkins, B. (1995).
- DNA Processing in Bacterial Conjugation.
Annual Rev Biochem, 64, 141-69.
